Home Page   -   ditorial Board   -   Instructions to autors   -   Archive   -   Contact   -   Journal's Indexing UA   EN


Last issue

3 (92), 2019


Support

Edition of the journal is supported partially by the grant of the Fogarty International Center (FIC) of the National Institute of Health and Illinois University, Chicago.

JOURNAL

ISSN 2077-7477 (Print)
ISSN 2077-7485 (Online)

A journal "Dovkillia ta zdorovia" (Environment & Health) publishes the articles on the problems in the field of medical ecology, hygiene, health protection and ecological safety.

Founder of the Journal:
State Institution "O.M. Marzeiev Institute for Hygiene and Medical Ecology of the National Academy of Medical Sciences of Ukraine"

Frequency of publication:
quarterly

Environment & HealthISSN: 2077-7477 eISSN: 2077-7485
No: 4 (80)   -   2016   -   Pages: 19-22
Assessment of cytotoxic activity of water from various water supply sources
Verholias M.R.1, Trakhtenberg I.M.2, Dmytrukha N.N.2
1 Dumansky A.V. Institute of Colloid Chemistry and Water Chemistry, National Academy of Medical Sciences of Ukraine, Kyiv
2 State Institution Institute of Occupational Medicine, National Academy of Medical Sciences of Ukraine, Kyiv

: 574.64:57.085.23

ABSTRACT:
Objective. We assessed a quality of drinking water from various sources of water supply by its cytotoxic effect on the cultures of human and animal cells in the experiments in vitro.

Materials and methods. We investigated a cytotoxic effect of control water, obtained according to the requirements of the State Standards of Ukraine 4174: 2003 in the Laboratory of the Institute of Colloid Chemistry and Water Chemistry, National Academy of Sciences of Ukraine, tap water, pump-room water, and bottled water "Znamenska." Studies were performed on the cells of HEK-293 line (human kidney cells), L929 line (mouse fibroblasts), and RTR (piglet testicles cells) from Zabolotny D.K. Institute of Microbiology and Virology, National Academy of Sciences of Ukraine.

Results. Research of cytotoxicity of mentioned water samples in the MTT-test showed that the most toxic activity against human kidney cells (HEK-293) was manifested by the pump room water and tap water (a number of viable cells under their effect made up 64.41% and 77.00% respectively). In "Znamenska" water a number of viable cells made up 81.75%, control water demonstrated the smallest impact -91.73%. In the test with sulforodamin B a number of living cells under exposure of control water made up 103.00%, of tap water - 82.45%, of water from the pump rooms - 89.20%, of "Znamenska" water - 96.75%. In the test with sulforodamin B after addition of control water a number of viable cells was 81.45%, after addition of "Znamenska" water - 69.45%, tap water - 68.2%, and water from the pump room - 66 95%.

Conclusions. Tap water had the highest cytotoxic effect according to MTT-test and test with sulforodamin B on human kidney cells (HEK-293), water from the pump room had the highest effect on the mouse fibroblast (L 929), water from the pump room had the highest one on the RTR testicles of piglets. Control water had the smallest impact on the cell viability. A negative impact of water on cell viability was manifested in the violations of mitochondria function and protein synthesis. The cells of RTR line were more sensitive to the toxic effect of water that may indicate the possible negative impact of water on the reproductive system of the organism.

KEYWORDS:
cytotoxicity, cell culture, drinking water.
REFERENCES:
1. Serdiuk A.M. Dovkillia ta zdorovia. 1998 ; 4 (7) : 2-6 (in Ukrainian).
2. Vergolias M. R., Goncharuk V.V. Khimiia i tekhnologiia vody. 2016; 38 (1) : 108-118 (in Russian).
3. Trakhtenberh I.M., Kovalenko V.M., Kokshareva N.V., Zhminko P.H., Chumak V.T. Alternatyvni metody i test-systemy [Alternative Methods and Test Systems]. Kyiv : Avitsenna; 2008 : 268 p. (in Ukrainian).
4. Goncharuk V.V., Vergolias M.R., Boltina I.V. Khimiia i tekhnologiia vody. 2013 ; 5 : 426-435 (in Russian).
5. Clemedson C., Blaauboer B., Castell J., Prieto P., Risteli L., Vericat J.-A., Wendel A. ALTEX. 2006 ; 23 (suppl.) : 254-258.
6. Combes R.D. Altern. Lab. Anim. 2004 ; 32 : 43-49. https://doi.org/10.1177/026119290403201s08
7. Trakhtenberh I.M., Ulberh Z.R., Chekman I.S., Dmytrukha N.M. et al. Metodychni rekomendatsii. Otsinka bezpeky likarskykh nanopreparativ [Methodical Recommendations. Assessment of the Safety of Medicinal Nanopreparations ]. Kyiv ; 2011 : 108 p. (in Ukrainian).
8. Yakubchak O.M., Zahrebelnyi V.O., Adamenko L.V. Shchodo toksychnosti khlorovanoi vodoprovidnoi vody [On the Toxicity of Chlorinated Tap Water]. In : Naukovyi visnyk Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy. Ser.: Veterynarna medytsyna, yakist i bezpeka produktsii tvarynnytstva [Scientific Bulletin of the National University of Bioresources and Nature Management of Ukraine. Ser.: Veterinarian Medicine, Quality, and Safety of Cattle-Breeding Production]. 2013 ; 188 (3) : 182-185 (in Ukrainian).
Designed by: IPH NAMSU Environment & Health, 2006-2019. All rights reserved.
Use of text and graphic materials of the site is allowed only upon written permission of the editoral board.