Довкілля та здоров'я

Home Page - Еditorial Board - Instructions to autors - Archive - Contact - Journal's Indexing

UA EN

Last issue

№ 1 (110), 2024

Support

Edition of the journal is supported partially by the grant of the Fogarty International Center (FIC) of the National Institute of Health and Illinois University, Chicago.

JOURNAL

ISSN 2077-7477 (Print)
ISSN 2077-7485 (Online)

A journal "Dovkillia ta zdorovia" (Environment & Health) publishes the articles on the problems in the field of medical ecology, hygiene, health protection and ecological safety.

Founder of the Journal:
State Institution "O.M. Marzeiev Institute for Hygiene and Medical Ecology of the National Academy of Medical Sciences of Ukraine"

Frequency of publication:
quarterly

Environment & Health

ISSN: 2077-7477 eISSN: 2077-7485

No: 1 (94) - 2020 - Pages: 39-47

Download Full Text (PDF)

Sensitivity to synthetic aromacyclic amino spirits of Candida isolated in the patients with type i diabetes

Osypchuk N.O.¹, Nastenko V.B.¹, Shyrobokov V.P.¹, Korotkyi Yu.V.²

¹ Department of Microbiology, Virology and Immunology, O.O. Bohomolets National Medical University, Kyiv
² Institute of Organic Chemistry, NASU, Kyiv

УДК: 582.282.23:57.017.4:541.4:616.37-008.64

https://doi.org/10.32402/dovkil2020.01.039

ABSTRACT:

The derivatives of aromatic amino spirits are a promising group of the substances with antimycotic effect.

Objective: The aim of the study was to investigate the sensitivity of clinical isolates of yeast-like fungi of Candida genus, obtained from oral subbiotopes of the patients with type I diabetes, to synthetic aromacyclic amino spirits and commercial antimycotics.

Materials and methods: We applied the bibliosemantic, microscopic, mycological, statistical and analytical methods in the study.

Results and discussion: 90 isolates of the representatives of Candida genus were isolated. The frequency of the isolation of yeast fungi in the oral biotope made up 66.1%. The level of oral candidiasis among patients with type I diabetes was 5.1%. C.albicans, C. glabrata, C. krusei, C. tropicalis, С. kefyr were indicated in the subbiotopes of oral cavity among the representatives of Candida genus, C. Albicans takes 67.8% , C. Glabrata - 10.0% , 16 , C. Krusei - 7% -, C. Tropicalis – 3.3%, S. kefyr - 2,2%. Among the compounds, Kc22 had the highest activity, which, at the concentration of 0.24-0.97 µg / ml, inhibited 59.0% of the strains. Amphotericin B showed the highest activity among commercial antimycotics. Fluconazole and intraconazole were ineffective against clinical strains.

Conclusions: The frequency of the isolation of yeast fungi in the oral biotope in the patients with type I diabetes made up 66.2%. C. albicans is a predominant candid in all studied oral subbiotopes (67.8%). Clinical isolates are resistant to itraconazole (100%) and fluconazole (81.7%). All isolates were sensitive to amphotericin B (75.3%). Alkyl (aryloxyethoxy) dialkyl-aminopropanol derivatives had high anticandidous activity against all types of clinical isolates.

KEYWORDS:

candidiasis, Candida, amino spirits, itraconazole, fluconazole, amphotericin B

REFERENCES:

1. Rodrigues C., Rodrigues M. and Henriques M. Candida sp. Journal of Clinical Medicine. 2019 ; 8 (1) : 76. DOI : https://doi.org/10.3390/jcm8010076

2. Karaa A. and Goldstein A. Pediatric Diabetes. 2015 ; 16 (1) ; 1–9. DOI : https://doi.org/10.1111/pedi.12223

3. King H., Aubert R. and Herman W. Diabetes Care. 1998 ; 21 (9) : 1414–1431. DOI : https://doi.org/10.2337/diacare.21.9.1414

4. Agarwal S., Raman R., Paul P., Rani P., Uthra S., Gayathree R., McCarty C. et al. Ophthalmic Epidemiology. 2005 ; 12 (2) : 143–153. DOI : https://doi.org/10.1080/09286580590932734

5. Guimarães T., Nucci M., Mendonça J., Martinez R., Brito L., Silva N., Moretti M. et al. International Journal of Infectious Diseases. 2012 ; 16 (6) : 442–447. DOI : https://doi.org/10.1016/j.ijid.2012.02.005

6. Khosravi A., Yarahmadi S., Baiat M., Shokri H. and Pourkabireh M. Journal de Mycologie Médicale. 2008 ; 18 (2) : 83–88. doi: https://doi.org/10.1016/j.mycmed.2008.04.002

7. Tang H., Liu W., Lin H. and Lai C. Geriatrics Gerontologie International. 2015 ; 15 (6) : 688–693. DOI : https://doi.org/10.1111/ggi.12329

8. Belazi M., Velegraki A., Fleva A., Gidarakou I., Papanaum L., Baka D. et al. Mycoses. 2005 ; 48 (3) : 192–196. DOI : https://doi.org/10.1111/j.1439-0507.2005.01124.x

9. Gonçalves R., Miranda E., Zaia J. and Giannini M. Mycopathologia. 2006 ; 162 (2) : 83–89. DOI : https://doi.org/10.1007/s11046-006-0038-5

10. Levytskyi A.P., Nikolyshyn A.K, Stupak O.P. and Stupak P.P. Ukrainskyi stomatolohichnyi almanakh. 2010 ; 2 (2) : 22-26 (in Ukrainian).

11. Wiederhold N., Tam V., Chi J., Prince R., Kontoyiannis D. and Lewis R. Antimicrobial Agents and Chemotherapie. 2006 ; 50 (2) : 469-473. DOI : https://doi.org/10.1128/AAC.50.2.469-473.2006

12. Berkow E. and Lockhart S. Infection and Drug Resistance. 2017 ; 10 : https://doi.org/237–245. DOI : https://doi.org/10.2147/IDR.S118892

13. Kondoh O., Inagaki Y., Fukuda H., Mizuguchi E., Ohya Y., Arisawa M., Shimma N. et al. Biological and Pharmaceutical Bulletin. 2005 ; 8 (11) ; 2138-2141. DOI : https://doi.org/10.1248/bpb.28.2138

14. Arendrup M., Cuenca-Estrella M., Lass-Florl C. and Hope W. Clinical Microbiologie and Infection. 2012 ; 18 (7) : 246-247. DOI : https://doi.org/10.1111/j.1469-0691.2012.03880.x

Download Full Text (PDF)