|Environment & Health||ISSN: 2077-7477 eISSN: 2077-7485
No: 1 (94) - 2020 - Pages: 39
Sensitivity to synthetic aromacyclic amino spirits of candida isolated in the patients with type i diabetes
Osypchuk N.O.1, Nastenko V.B.1, Shyrobokov V.P.1, Korotkyi Yu.V.2
1 Department of Microbiology, Virology and Immunology, O.O. Bohomolets National Medical University, Kyiv
2 Institute of Organic Chemistry, NASU, Kyiv
The derivatives of aromatic amino spirits are a promising group of the substances with antimycotic effect.
Objective: The aim of the study was to investigate the sensitivity of clinical isolates of yeast-like fungi of Candida genus, obtained from oral subbiotopes of the patients with type I diabetes, to synthetic aromacyclic amino spirits and commercial antimycotics.
Materials and methods: We applied the bibliosemantic, microscopic, mycological, statistical and analytical methods in the study.
Results and discussion: 90 isolates of the representatives of Candida genus were isolated. The frequency of the isolation of yeast fungi in the oral biotope made up 66.1%. The level of oral candidiasis among patients with type I diabetes was 5.1%. C.albicans, C. glabrata, C. krusei, C. tropicalis, Ñ. kefyr were indicated in the subbiotopes of oral cavity among the representatives of Candida genus, C. Albicans takes 67.8% , C. Glabrata - 10.0% , 16 , C. Krusei - 7% -, C. Tropicalis – 3.3%, S. kefyr - 2,2%. Among the compounds, Kc22 had the highest activity, which, at the concentration of 0.24-0.97 µg / ml, inhibited 59.0% of the strains. Amphotericin B showed the highest activity among commercial antimycotics. Fluconazole and intraconazole were ineffective against clinical strains.
Conclusions: The frequency of the isolation of yeast fungi in the oral biotope in the patients with type I diabetes made up 66.2%. C. albicans is a predominant candid in all studied oral subbiotopes (67.8%). Clinical isolates are resistant to itraconazole (100%) and fluconazole (81.7%). All isolates were sensitive to amphotericin B (75.3%). Alkyl (aryloxyethoxy) dialkyl-aminopropanol derivatives had high anticandidous activity against all types of clinical isolates.
candidiasis, Candida, amino spirits, itraconazole, fluconazole, amphotericin B
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