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Edition of the journal is supported partially by the grant of the Fogarty International Center (FIC) of the National Institute of Health and Illinois University, Chicago.

JOURNAL

ISSN 2077-7477 (Print)
ISSN 2077-7485 (Online)

A journal "Dovkillia ta zdorovia" (Environment & Health) publishes the articles on the problems in the field of medical ecology, hygiene, health protection and ecological safety.

Founder of the Journal:
State Institution "O.M. Marzeiev Institute for Hygiene and Medical Ecology of the National Academy of Medical Sciences of Ukraine"

Frequency of publication:
quarterly

Environment & HealthISSN: 2077-7477 eISSN: 2077-7485
No: 3 (83)   -   2017   -   Pages: 9-13
Methodical approaches to the assessment of toxicity and hazard of nanomaterials
Leonenko N.S.1, Demetska O.V.1, Leonenko O.B.1
1 SI "Institute of Occupational Medicine, NAMSU, Kyiv

: [(54-148:543.3):615.9]:005

ABSTRACT:
Background: Despite the large number of test systems for screening assessment of the impact of nanoscale substances on the organism, a choice of the most sensitive ones, depending on the objectives and tasks of the study, is a high-priority task.

Objective: We studied a toxicity of some nanomaterial (NM) samples by a combined indicator of the effect of general toxicity on the short-term culture of the bull's spermatozoa and generalization of the world experience in assessment of the toxicity and hazard of nanoparticles (NPs) and NMs.

Materials and methods: We investigated a cytotoxicity of nanoparticles (NPs) of metals (Mn, Co, Mg, Zn, Fe, Mo, Au) in the citrate solution. Particle sizes were measured by the analyzer Fritch (Germany). Toxicity assessment was performed on the AT-05 analyzer.

Results: NPs of molybdenum, iron, magnesium, manganese, and cobalt didnt show a cytotoxic effect in the native solution but gold NP demonstrated the cytotoxic effect (It, = 20.2%). In repeat study they demonstrated the cytotoxic effect in the concentrations of 510-3 mol/l (It, = 44.2%), 110-3 mol/l (It, = 68.4%) and the cytotoxic effect wasnt detected only in the concentration of 110-4 mol/l (It, = 100%). In many respects this can be due to the physical-and- chemical properties of gold NPs and size which was less in comparison with other studied metals.

Conclusions: Application of the alternative methods is necessary for screening assessment of the NM toxicity or nanoproducts. There is an urgent need to improve the scientific approaches to the assessment of their toxicity and hazard.

KEYWORDS:
nanomaterials, nanoparticles, toxicity, hazard
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