Objective: determination of the nature and features of changes in biochemical indicators in the blood of animals under conditions of chronic exposure to various concentrations of chlorites in drinking water to substantiate the criteria for hygienic evaluation of their adverse effects on the body.
Materials and methods: isolated effect of different concentrations of chlorites in drinking water on biochemical parameters.
Results: It was established that the consumption of drinking water with a chlorite content of 0.2 mg/dm3 does not affect the level of biochemical indicators in the blood of experimental animals. Chlorites in concentrations of 0.7 and 1.2 mg/dm3 cause more pronounced changes in metabolic parameters over time. The analysis of the obtained results of studies of integral and specific biochemical indicators shows that with long-term consumption of drinking water with chlorite content in concentrations of 0.7 and 1.2 mg/dm3, an increased content of total protein in blood serum is observed, which may indicate a negative effect of chlorite on processes synthesis. At the same time, compensatory reactions are manifested by shifts in bioenergetic processes and redistribution of metabolic energy-supplying pathways, such as anaerobic glycolysis, with the inclusion of energy resources of creatinine. Load with doses of 0.7 and 1.2 mg/dm3 led to stress at the cellular level, and the mobilization of compensatory mechanisms occurs at the level of metabolism of organ-specific enzymes.
It was established that the nature and features of biochemical changes in the blood of experimental animals depended on the concentration of chlorite and the time of action.
In the conditions of a chronic experiment, changes in biochemical indicators that do not go beyond physiological deviations of the norm have a stable and permanent character, which is ensured by the development of non-specific protection mechanisms and in this aspect can be considered as adaptive.
Conclusions. The nature and peculiarities of changes in biochemical indicators in the body of experimental animals during a six-month chronic sanitary-toxicological experiment with the isolated effect of different concentrations of chlorites in drinking water may indicate a violation of metabolism in organs and changes in the intensity of regenerative processes at the cellular level in response to the adverse effects of toxicants. The established biochemical changes in the blood of the experimental animals are small compared to the control, statistically reliable within the physiological norm and had a constant character, may indicate the development of adaptation processes in the body under the action of chlorites in drinking water.

drinking water, chlorites, isolated action, biochemical indicators

1. Prokopov VO, Lypovetska OB, Kulish TV, Kostiuk VA, Avramenko LP. Nebezpechni khloryty u pytnii vodi: utvorennia ta vydalennia z vykorystanniam dioksydu khloru u tekhnolohii vodopidhotovky [Hazardous chlorites in drinking water: formation and removal when using chlorine dioxide in water preparation technology]. Dovkillia ta zdorovia [Environment & Health]. 2023 Mar ;(1 (106)):43-50. Ukrainian https://doi.org/10.32402/dovkil2023.01.043

2. Mokiienko A, Bondarchuk O, Spasonova L. Obhruntuvannia bezpechnosti khlorytiv ta khlorativ yak pokhidnykh dioksydu khloru pry ochyshchenni ta znezarazhenni pytnoi vody [Justification of the safety of chlorites and chlorates as derivatives of chlorine dioxide in the purification and disinfection of drinking water]. In: Chysta voda. Fundamentalni, prykladni ta promyslovi aspekty [Clean water. Fundamental, applied and industrial aspects: conf. papers; 2023 Nov 9-10]. Kyiv; 2023. P. 118-21. Ukrainian

3. Directive (EU) 2020/2184 of the European Parliament and of the council of 16 December 2020 on the quality of water intended for human consumption (recast). Official Journal of the European Union. 2020 Dec 23;(L 62.435/1):1

4. Pro zatverdzhennia Zmin do dodatku 2 do Derzhavnykh sanitarnykh norm ta pravyl «Hihiienichni vymohy do vody pytnoi, pryznachenoi dlia spozhyvannia liudynoiu» [On approval of Amendments to Appendix 2 to the State Sanitary Norms and Rules «Hygienic Requirements for Drinking Water Intended for Human Consumption»: Order of the Ministry of Health of Ukraine № 341; 2022 Feb 18]. Ukrainian https://zakon.rada.gov.ua/laws/show/z0304-22#Text

5. Pro zatverdzhennia Derzhavnykh sanitarnykh norm i pravyl «Pokaznyky bezpechnosti ta okremi pokaznyky yakosti pytnoi vody v umovakh voiennoho stanu ta nadzvychainykh sytuatsiiakh inshoho kharakteru» [On approval of the State Sanitary Norms and Rules «Safety Indicators and Certain Indicators of Drinking Water Quality in Conditions of Martial Law and Other Emergency Situations» : Order of the Ministry of Health of Ukraine № 683, 2022 Apr 22]. Ukrainian https://zakon.rada.gov.ua/laws/show/z0564-22#Text

6. European Convention for the Protection of Vertebrate Animals Used for Experiments or for Other Scientific Purposes. Council of Europe. 1986 Mar 18.

7. Pro zatverdzhennia Poriadku provedennia naukovymy ustanovamy doslidiv, eksperymentiv na tvarynakh [Procedure for Conducting Experiments and Experiments on Animals by Scientific Institutions: Order of the Ministry of Education and Science, Youth and Sports of Ukraine № 249, 2012 Mar 1]. Ukrainian https://zakon.rada.gov.ua/laws/show/z0416-12#Text

8. Antomonov M, Korobeinikov H, Khmelnytska I, Kharkovliuk-Balakina N. Matematychni metody obroblennia ta modeliuvannia rezultativ eksperymentalnykh doslidzhen [Mathematical methods for processing and modeling the results of experimental research]. Kyiv : Olimpiiska literature; 2021. 216 p. Ukrainian

9. Kravchun TYe, Tomashevska LA, Prokopov VO, Kulish TV, Tsytsyruk VS. Osoblyvosti systemy krovi tvaryn za umov spozhyvannia pytnoi vody zi vmistom khlorativ [Peculiarities of the blood system of animals under the conditions of consumption of drinking water containing chlorates]. Medicni perspektivi [Medical Perspectives]. 2024 Jun 28; 29(2):187-93. Ukrainian https://doi.org/10.26641/2307-0404.2024.2.307693