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2 (103), 2022


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Edition of the journal is supported partially by the grant of the Fogarty International Center (FIC) of the National Institute of Health and Illinois University, Chicago.

JOURNAL

ISSN 2077-7477 (Print)
ISSN 2077-7485 (Online)

A journal "Dovkillia ta zdorovia" (Environment & Health) publishes the articles on the problems in the field of medical ecology, hygiene, health protection and ecological safety.

Founder of the Journal:
State Institution "O.M. Marzeiev Institute for Hygiene and Medical Ecology of the National Academy of Medical Sciences of Ukraine"

Frequency of publication:
quarterly

Environment & HealthISSN: 2077-7477 eISSN: 2077-7485
No: 2 (103)   -   June, 2022   -   Pages: 4-9
Assessment of the potential toxicity of detergents with disinfectants properties on cell culture in vitro
Dmytrukha N..1, Korolenko T.K.1, Lahutina O.S.1, Lehkostup L.A.1
1 State Institution Kundiiev Institute of Occupational Health of the National Academy of Medical Sciences of Ukraine, Kyiv

: 57.086.83:614.48

ABSTRACT:
Detergents with disinfectants properties today are widely used in various fields of human activity for cleaning surfaces, equipment, tools while destroying infectious agents. An active substance, as disinfectants, it contains various chemical compounds with antimicrobial, antiviral and antifungal properties. To establish the safety of these substances, along with toxicological studies on animals, experiments are conducted on alternative in vitro test systems, in particular on cell culture, which allows to quickly assess and predict the potential toxicity of detergents to humans and other living organisms.

The purpouse of the study was assessment of the potential danger of detergents and disinfectants for humans by determining their cytotoxic effects in experiments on cell culture in vitro.

Material and methods. Studies were performed to evaluate the cytotoxic effect of detergents with disinfectants properties : "Bioblysk", "Blysk Plus" and "Blysk" (manufactured by PE "HYGIENICS", Poland) on the lung cancer cells (line A-549) using cytochemical methods: MTT- with methyltetrazolium blue and CRB - test with sulforodamine B.

Results. It was found that all detergents have the greatest cytotoxic effect on A-549 cells at a concentration of 1.0% of the native solution. The lowest concentrations 0.03% and 0.015% have no toxic effect on cells. With the help of probit analysis based on the obtained results, LC50 values were calculated: for Bioblysk - 0.15% (MTT test) and 0.15% (CRP test); for Blysk Plus LC50 - 0.25% (MTT test) and 0.2% (CRP test); for Blysk - 0.2% (MTT test and CRP test). LC50 values for detergents were 10 times lower than the recommended minimum concentrations of working solutions

Conclusions. The obtained data indicate that the citotoxicity effect of detergents on A-549 cells was manifested by the effect on mitochondrial function and protein synthesis. The severity of studied drugs toxic effect depended on its chemical composition and pH, concentration in the incubation medium. The calculated LC50 values indicate their possible danger to the respiratory system, so they require appropriate protection.

KEYWORDS:
detergents and disinfectants, cytotoxicity, cell culture
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