Substantiation of safe use of disinfectant «STERIZENT»

Abstract

diseases, especially a use of the disinfectants (DS), in particular, are of special relevance in order to break the mechanisms of the transmission of infectious agents. The variety of DS, presented at the modern market, allows us to choose the most appropriate ones for the purposes, conditions and methods of application. However, the effectiveness and safety of the DS both for humans and the environment are still the main choice criteria. In this regard, electrolysis preparations (anolytes) attract a special attention. Objective: We determined the bactericidal, virucidal and yeast activity of the STERISENT preparation (Israel) according to modern requirements and standards. Materials and methods: Studies of the specific activity of the DS "STERISENT" were carried out in accordance with the Order of the Ministry of Health of Ukraine No. 2024, 03.09.2020 and the European standards. The test cultures and culture media recommended by the Order No. 2024 and the standards EN 13727: 2012 + A2: 2015 [3] and DSTU EN 13624: 2019 (EN 13624: 2013, IDT) were used in the quantitative suspension method. Virucidal activity was studied in the intestinal bacteriophages T2 and MS2.Toxicological studies were carried out in the laboratory animals in accordance with modern standards. Results: A high bactericidal and yeast-killing activity of STERISENT preparation (Israel) in undiluted form was established for Escherichia coli K12 NCTC 10538 lg R >5.42, for Pseudomonas aeruginosa lg R >5.37; for Staphylococcus aureus lg R >5.34; for Candida albicans lg R >4.31at exposure of 30 s and 60 s. In the study in bacteriophages at an exposure of 30 s, the logarithms of the reduction were: T2 - >5.08, MS2 - >5.12. According to GOST 12.1.007-76,by the parameters of acute toxicity, STERIZENT preparation belongs to class 4 - low-hazardous substances when administered to the stomach and applied to the skin; at the inhalation application in saturated concentrations - to class 3. moderately dangerous substances. The preparation does not have a sensitizing effect in the recommended modes of application. Conclusions: The obtained results make it possible to recommend the STERISENT preparation (Israel) for wide practical use as a highly effective and one of the least toxic DS.