Folate cycle genetics, level of homocysteine, thyroid and pituitary hormones in the blood among the children from the districts bordering the Chornobyl exclusion zone

Ю.І. Бандажевський; Н.Ф. Дубова

doi:10.32402/dovkil2021.03.030

Authors

Yu.I. Bandazhevskyi Ecology and Health Coordination and Analytical Centre, Ivankiv Author
N.F. Dubova P. L. Shupyk National Medical Academy for Post-Graduate Education, Kyiv Author

DOI:

https://doi.org/10.32402/dovkil2021.03.030

Keywords:

homocysteine, folate cycle, thyroid and pituitary hormones, adolescents, radiation contaminated areas

Abstract

Objective: The aim of the study was to identify interrelations between the state of folate cycle genetic apparatus and blood levels of homocysteine, thyroid and adenohypophysis hormones in the blood among the children from Ivankivskyi and Poliskyi districts, Kyiv region, bordering the Chornobyl exclusion zone (СhEZ). Methods: Immunochemical, genetic, statistical ones. Results: We measured blood levels of homocysteine (Hcy), pituitary thyroid-stimulating hormone (TSH), free triiodothyronine (T3), free thyroxine (T4) in 158 children from Poliskyi district and 178 children from Ivankivskyi district and assessed the state of a genetic system of the folate cycle (FC). The average age of the examined was (15.2 ± 0.9) years old (95% CI 15.0-15.4 years old). Taking into account the FC genotypes, genetic subgroups were formed, they were based on 100% representation of one specific genotype. The laboratory examination was carried out twice: in April and December 2015 (before and after the fires in the ChEZ). It was found out that in the total group of the children, as well as in most of analyzed genetic subgroups, the level of Hcy and thyroid hormones (TG) in the children from Ivankivskyi district was statistically significantly higher than in those from Poliskyi district. At the same time, a direct association between Hcy and TSH was observed in the total group, as well as in most of the genetic subgroups of children from Ivankivskyi district, and this ssociation was absent in the children from Poliskyi district. The blood level of Hcy both in the children from Poliskyi district and in the children from Ivankivskyi district was statistically significantly higher in the subgroup containing only homozygous variants of the T allele of the MTHFR:677 genetic polymorphism in comparison with the subgroups containing neutral C alleles of the same polymorphism. At the same time, there were no differences for TSH, T3 and T4. Сonclusions: The analysis enabled to establish a synchronous reaction of metabolic cycles ensuring the metabolism of Hcy and thyroid hormones in the adolescents, regardless of the FC genotypes. Correlation analysis, as well as the results of statistical analysis, indicate that an increase in the level of Hcy in the blood of children induced the synthesis of TSH and T3. The results show a close relationship between thyroid hormone genesis and the metabolism of methionine sulfur-containing amino acids and Hcy. A forest fire, containing long-lived radioactive elements, is the most likely reason for the increase of Hcy level and, as a result, the hormones of the pituitary-thyroid axis in the blood of the children living in the districts, bordering the ChEZ.

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References

1. BandazhevskyYu.I., Dubova N.F. Сomparative assessment of metabolic processes in children living in the areas affected by the Chоrnobyl Nuclear Power plant accident. Довкілля та здоров’я. 2017. № 4. С. 27-30. https://doi.org/10.32402/dovkil2017.04.027

2. Mc Cully KS. Homocysteine and the pathogenesis of atherosclerosis. Expert Review of Clinical Pharmacology. 2015. Vol. 8 (2). P. 211-219. https://doi.org/10.1586/17512433.2015.1010516

3. Lai W.K., Kan M.Y. Homocysteine-induced endothelial dysfunction. Annals of Nutrition and Metabolism. 2015. Vol. 67 (1). P. 1-12. https://doi.org/10.1159/000437098

4. Ganguly P., Alam S. Role of homocysteine in the development of cardiovascular disease. Nutrition Journal. 2015. № 14. 6 р. https://doi.org/10.1186/1475-2891-14-6

5. Полушин А.Ю., Одинак М.М., Янишевский С.Н., Голохвастов С.Ю., Цыган Н.В. Гипергомоцистеинемия – предиктор тяжести инсульта на фоне обширности повреждения мозгового вещества. Вестник Российской военно-медицинской академии. 2013. № 4 (44). С. 89-94.

6. Varshney K.K., Gupta J.K., Mujwar S. Homocysteine induced neurological dysfunctions: A link to neurodegenerative disorders. International Journal of Medical Research & Health Sciences. 2019. Vol. 8. № 4. P. 135-146.

7. Keshteli A., Baracos V., Madsen K. Hyperhomocysteinemia as a potential contributor of colorectal cancer development in inflammatory bowel diseases: A review. World J Gastroenterol. 2015. Vol. 21(4). P. 1081–1090. https://doi.org/10.3748/wjg.v21.i4.1081

8. Тепляков А.Т., Березикова Е.Н., Шилов С.Н., Гракова Е.В., Торим Ю.Ю., Ефремов А.В. и др. Оценка роли гипергомоцистеинемии и полиморфизма С677Т гена метилентетрагидрофолат-редуктазы в развитии хронической сердечной недостаточности. Кардиоваскулярная терапия и профилактика. 2016. T. 15 (4). C. 22-28. https://doi.org/10.15829/1728-8800-2016-4-22-28

9. Бандажевский Ю. И., Дубовая Н. Ф. Роль генома фолатного цикла в возникновении гипергомоцистеинемии у детей из районов, пострадавших в результате аварии на Чернобыльской атомной электростанции. Чорнобиль: екологія і здоров’я: науково-практичний збірник ; за заг. ред. Ю.І. Бандажевського. Іванків : ПУ Координаційний аналітичний центр «Екологія і здоров’я» ; Дніпро : Середняк Т.К., 2017. Вип. 5. С. 26-35.

10. Catargi B., Parrot-Roulaud F., Cochet C. et al. Homocysteine, hypothyroidism, and effect of thyroid hormone replacement. Thyroid. 1999. Vol. 9 (12). P. 1163-1166. https://doi.org/10.1089/thy.1999.9.1163

11. Загальнодозиметрична паспортизація та результати ЛВЛ-моніторингу у населених пунктах України, які зазнали радіоактивного забруднення після Чорнобильської катастрофи. Дані за 2011 р. ЗБІРКА 14. К. : МОЗ України, НАМНУ України, МНС України, ДАЗВ, ДУ «ННЦРМ НАМН України», НД ІРЗ АТН України, 2012. 99 с.

12. Бандажевський Ю.І., Дубова Н.Ф. Пожежі лісу в Чорнобильській зоні відчуження та здоров’я дітей. Іванків: ПУ Координаційний аналітичний центр «Екологія і здоров’я»; Дніпро: Середняк Т.К., 2020. 42 с.

13. Labunska I., Levchuk S., Kashparov V. et al. Current radiological situation in areas of Ukraine contaminated by the Chornobyl accident: Part 2. Strontium-90 transfer to culinary grains and forest woods from soils of Ivankiv district. Environment International. 2021. Vol. 146. 106282. DOI: https://doi. org/10.1016/j.envint.2020.106282

14. Кашпаров В.А., Миронюк В.В., Журба М.А., Зибцев С.В., Глуховский А.С., Жукова О.М. Радиологические последствия пожара в Чернобыльской зоне отчуждения в апреле 2015 года. Радиационная биология. Радиоэкология. 2017. Т. 57. № 5. С. 512-527.

15. Sheybak V.M., Lelevich V.V., Bandazhevskyі Yu.I. Свободные аминокислоты плазмы крови и тканей животных после инкорпорирования радионуклидов цезия и стронция. Клинико-экспериментальные аспекты влияния инкорпорированных радионуклидов на организм / под ред. Ю.И. Бандажевского и В.В. Лелевича. Гомель, 1995. P. 83-106.

16. Колпакова А.Ф., Шарипов Р.Н., Волкова О.А. Влияние загрязнения атмосферного воздуха взвешенными веществами на сердечно-сосудистую систему. Сибирский медицинский журнал. 2015. Т. 30. № 3. С. 7-12.

17. Pope C.A., Turner M.C., Burnett R., Jerrett M., Gapstur S.M., Diver W.R. et al. Relationships between fine particulate air pollution, cardiometabolic disorders and cardiovascular mortality. Circ. Res. 2015. Vol. 116, № 1. P. 108-115. https://doi.org/10.1161/CIRCRESAHA.116.305060

18. Park S.K., O’Neill M.S., Vokonas P.S., Sparrow D., Spiro A. 3-rd, Tucker K.L. et al. Traffic-related particles are associated with elevated homocysteine. The VA Normative Aging Study. Am. J. Respir. Crit. Care Med. 2008. Vol. 178. P. 283-289. https://doi.org/10.1164/rccm.200708-1286OC